Screening for potential antimicrobial properties from oil palm root against Ganoderma boninense

Chong, Khim Phin Screening for potential antimicrobial properties from oil palm root against Ganoderma boninense. (Unpublished)

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Abstract

The role of phenolics in oil palm root resistance mechanism against G. boninense was studied. High Performance Liquid Chromatography (HPLC) injection with standards and LCMS/Q-tof identified the presence of three phenolics; syringic acid, caffeic acid and 4-hydroxybenzoic acid (HBA) in oil palm roots which contributed to the defence mechanism against G. boninense. A VROS showed a higher content of all these three phenolics compared to Ekona and Calabar. In vitro studies with phenolics incorporated either in 10% Potato Dextrose Agar (PDA), Oil Palm Root Agar (OPRA) or Oil Palm Root Broth (OPRB) revealed that the common concentration of syringic acid, caffeic acid and 4-HBA; ranging between 50-110 Ilg/g in oil palm root tissues failed to stop further invasion of G. boninense. However, addition of chitosan is potent in stimulating the continuous production of these phenolics in living oil palm roots, without addition of chitosan showed a lower accumulation of phenolics and higher disease severity. Higher concentrations of the phenolics, either syringic acid, caffeic acid or 4-HBA such as 90 and 110 Ilg also potentially inhibited the growth of this pathogen but not killing them. G. boninense was also found to be able to degrade all these phenolics to less toxic compounds.

Item Type: Research Report
Uncontrolled Keywords: G. boninense , HPLC , toxic compounds , acid
Subjects: Q Science > QK Botany
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Noraini
Date Deposited: 25 Aug 2020 03:24
Last Modified: 27 Oct 2020 06:11
URI: http://eprints.ums.edu.my/id/eprint/25837

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