Study of volatile compouds extraction using SPME-GCMS in local seaweeds of Kappaphycus alvarezii, Caulerpa lentilifera and Sagassum polycystum

Vijaya Ratinam A/L Ramah, (2009) Study of volatile compouds extraction using SPME-GCMS in local seaweeds of Kappaphycus alvarezii, Caulerpa lentilifera and Sagassum polycystum. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A pioneer study with a new extraction method was applied to the volatile compounds of three local seaweeds from Borneo, Cilulerpa lentillifera, Kappaphycus alvarezii and Sagassum polycystum using HS-SPME method. Dynamic headspace was optimized according to an experimental design, with appropriate temperature of 50°C and 60°C and extraction time as 20min, 30min, 40min and 50min. Two different fibres namely PDMS and PDMS-DVB were utilized in the extraction. The optimization result varies according to seaweeds with the temperature of 60°C and extraction time of 50 minutes for both fibres for K. alvarezii and PDMS fibre extraction for S. polycystum. While for PDMS-DVB extraction for the same seaweed spesies, a 50°C temperature with 20 minute extraction time provided optimum peak areas. Cilulerpa lentillifera shows peaks with highest abundance when extracted in 50°C with 20 minute for PDMS fibre and 50°C with 40 minute extraction for PDMS-DVB fibre. GC-MS analysis was performed on this extract to identify the volatile components. Significantly, PDMS fibre extracted more compounds than PDMS-DVB. Thus PDMS is recommended as a better fibre comparatively to PDMSDVB in volatile compounds extraction using SPME for these particular seaweeds. Qualitative study portray a total of 223 volatile constituents successfully extracted and identified with 82, 91 and 50 volatiles from each species of K. alvarezii, C lentillifera and S. polycystum respectively. The volatile compounds are comprised of nine chemical compounds of hydrocarbons, aldehydes, ketones, esters, alcohols, halogenated compounds, add compounds, aromatic compounds and some miscellaneous compounds. Comparison on the major volatile constituents among the seaweeds shows a similar percentage of volatile compounds in all seaweeds among the nine groups. Within them, hydrocarbon compounds were the most characteristic of all three algae, and more particularly, all three seaweeds mostly share the common constituents in their structures.

Item Type: Academic Exercise
Uncontrolled Keywords: Seaweeds, Local seaweeds, Kappaphycus alvarezii, Caulerpa lentillifera, Sagassum polycystum
Subjects: ?? SH393 ??
?? QK504-(638) ??
Divisions: SCHOOL > School of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 22 Jun 2012 01:20
Last Modified: 24 Oct 2017 06:58
URI: http://eprints.ums.edu.my/id/eprint/4352

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