A study on general semen characteristics of dorper ram and the effect of room and chilled temperature on sperm progressive motility

Siti Nur Nadia Abdul Razak, (2010) A study on general semen characteristics of dorper ram and the effect of room and chilled temperature on sperm progressive motility. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A study was conducted at Livestock Strategic Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kluang, Johore. The aims of this study were to observe the general semen characteristics of Dorper ram and the effect of storage at room and chilled temperature on sperm progressive motility. For this purpose, six Dorper rams were selected. All the rams were individually subjected to one week of study and were scheduled for semen collection twice weekly using a teaser ewe. In each session, the reaction time was recorded. Semen was collected using an artificial vagina (AV) and was evaluated for volume, gross wave motility sperm progressive motility, sperm concentration, live sperm and abnormal sperm Each semen sample was diluted with extender divided into two tubes. One tube was stored at 5°C and the other at 26°C for seven days. The mean of reaction time, semen volume, wave pattem, sperm progressive motility percentage, sperm concentration, live sperm and abnormal sperm were 13.3±1.9 sec, 1.03±0.1 ml, 4.0±0.4, 69.2±7.8%, 2.44±0.4 xl09 sperm/ml, 76.0±2.4% and 14.4±7.s%, respectively. On the third day of storage, it was observed that the semen that were stored at 26°C lost its progressive motility to 0% while semen stored at 5°C were observed with 50% progressive motility. The semen stored at 5°C lost its progressive motility to 0% on the sixth day of storage. It was concluded that the general semen characteristics of Dorper ram was within normal range and there was no significant difference on reaction time and semen quality between first and second collection. For the diluted semen, there was significant difference on sperm progressive motility percentage at 5°C in which the length of period for the sperm to lose its progressive motility percentage to 0% was longer compared to semen sample stored at 26°C.

Item Type: Academic Exercise
Uncontrolled Keywords: Dorper ram, Semen characteristics, Temperature, Sperm progressive motility
Subjects: S Agriculture > SF Animal culture
Divisions: SCHOOL > School of Sustainable Agriculture
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 08 Oct 2012 10:00
Last Modified: 13 Oct 2017 03:32
URI: http://eprints.ums.edu.my/id/eprint/5038

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