Production of hybrid Orchid: in vitro seed germination of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; And Vanda dearei x Vanda helvola

Fung, Joseph (2010) Production of hybrid Orchid: in vitro seed germination of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; And Vanda dearei x Vanda helvola. Universiti Malaysia Sabah. (Unpublished)

[img]
Preview
Text
ae0000000801.pdf

Download (2MB) | Preview

Abstract

A hybrid is a result of the union, whether naturally occurring or man made, of two plants of different genetic backgrounds. Assisted cross-pollination was carried out by placing the pollen of one orchid plant onto the stigma of another orchid plant, resulting in the formation of seeds which grow into a new hybrid. The in vitro seed germination of hybrid seeds of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; and V. dearei x V. helvola were done by using half strength MS medium supplemented with 10% v/v of coconut water, 10% w/v of tomato juice, 10% w/v of potato homogenate and a control treatment. For 30% germination, R. bella hybrid seeds took 10 days after culture on half-MS supplemented with coconut water, P. denevei hybrid 14 days after culture on half-MS without additive, and V. dearei hybrid took 14 days after culture on half-MS supplemented with potato homogenate. All cultures from the entire studies were maintain under 24 hour light conditions at 24±2 Dc. The analysis of variance (ANOVA) confirmed that there is a significant effect of complex additives on the in vitro germination of all the three hybrids seeds at 20-60 days after culture.

Item Type: Academic Exercise
Uncontrolled Keywords: Orchid, Hybrid, Genetic, Orchid plant, Renanthera bella x Vanda helvola, Paraphalaenopsis denevei x Phalaenopsis bellina, V. dearei x V. helvola
Subjects: ?? QK474.8-495 ??
Divisions: SCHOOL > School of Sustainable Agriculture
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 22 Oct 2012 08:04
Last Modified: 13 Oct 2017 04:10
URI: http://eprints.ums.edu.my/id/eprint/5099

Actions (login required)

View Item View Item

Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository