Production of hybrid Orchid: in vitro seed germination of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; And Vanda dearei x Vanda helvola

Fung, Joseph (2010) Production of hybrid Orchid: in vitro seed germination of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; And Vanda dearei x Vanda helvola. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A hybrid is a result of the union, whether naturally occurring or man made, of two plants of different genetic backgrounds. Assisted cross-pollination was carried out by placing the pollen of one orchid plant onto the stigma of another orchid plant, resulting in the formation of seeds which grow into a new hybrid. The in vitro seed germination of hybrid seeds of Renanthera bella x Vanda helvola; Paraphalaenopsis denevei x Phalaenopsis bellina; and V. dearei x V. helvola were done by using half strength MS medium supplemented with 10% v/v of coconut water, 10% w/v of tomato juice, 10% w/v of potato homogenate and a control treatment. For 30% germination, R. bella hybrid seeds took 10 days after culture on half-MS supplemented with coconut water, P. denevei hybrid 14 days after culture on half-MS without additive, and V. dearei hybrid took 14 days after culture on half-MS supplemented with potato homogenate. All cultures from the entire studies were maintain under 24 hour light conditions at 24±2 Dc. The analysis of variance (ANOVA) confirmed that there is a significant effect of complex additives on the in vitro germination of all the three hybrids seeds at 20-60 days after culture.

Item Type: Academic Exercise
Uncontrolled Keywords: Orchid, Hybrid, Genetic, Orchid plant, Renanthera bella x Vanda helvola, Paraphalaenopsis denevei x Phalaenopsis bellina, V. dearei x V. helvola
Subjects: ?? QK474.8-495 ??
Divisions: SCHOOL > School of Sustainable Agriculture
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 22 Oct 2012 08:04
Last Modified: 13 Oct 2017 04:10
URI: http://eprints.ums.edu.my/id/eprint/5099

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