Genetic diversity study on Gallus gallus var. Thailand indigenous chicken, Taiwan indigenous chicken and broiler based on GHR-intron 2

Lim, Lee Ging (2010) Genetic diversity study on Gallus gallus var. Thailand indigenous chicken, Taiwan indigenous chicken and broiler based on GHR-intron 2. Universiti Malaysia Sabah. (Unpublished)

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Abstract

It was known that growth of broiler is rapid than indigenous chicken. The growth rate (phenotype) is influenced by genotype and environment. The primary objective of the research was to study the diversity of growth related genes of Gallus gallus var. Thailand indigenous chicken, Taiwan indigenous chicken and broiler (Hubbard) based on GHR-Intron 2. First, blood samples of Thailand Indigenous Chicken, Taiwan Indigenous Chicken and broiler were collected. After that, DNA of blood of three different varieties was extracted. Then, PCR amplification was carried out by using GHR Intron-2. RFLP-PCR was followed after PCR amplification with the digestion of enzyme HindIII. The frequency of A�/A₂ alleles in the overall population was 0.2115 (A�) and 0.7885 (A₂) for GHR-Intron 2. The genotype frequency in overall population was 0.0447 (A�A�)' 0.3336 (A�A₂) and 0.6217 (A₂A₂). The chi square value (x²) for overall population is 42.98 with the probability 0.00. The observed heterozygosity and expected heterozygosity of overall population are 0.0909 and 0.3535, respectively. Low genetic diversity found with the genetic distance value of 0.0047 (Broiler/Thailand Indigenous Chicken), 0.0578 (BroilerfTaiwan Indigenous Chicken) and 0.0973 (Thailand/Taiwan Indigenous Chicken). The study found that indigenous chicken and broiler had no difference in GHR-Intron 2 gene.

Item Type: Academic Exercise
Uncontrolled Keywords: Gene, indigenous Chicken, Thailand indigenous, Gallus gallus var, GHR-intron 2
Subjects: ?? SF481-507 ??
Divisions: SCHOOL > School of Sustainable Agriculture
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 15 Oct 2012 01:51
Last Modified: 13 Oct 2017 04:11
URI: http://eprints.ums.edu.my/id/eprint/5102

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