Isozyme analysis and relationships among three species in Malaysian Trichoderma isolates

Shafiquzzaman Siddiquee, and Soon, Guan Tan and Umi Kalsom Yusof, (2010) Isozyme analysis and relationships among three species in Malaysian Trichoderma isolates. Journal of Microbiology and Biotechnology , 20 (9). pp. 1266-1275. ISSN 1266-1275

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Official URL: http://www.academia.edu/327336/Isozyme_Analysis_an...

Abstract

Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderma species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared to the other isolates of Trichoderma species.

Item Type:Article
Uncontrolled Keywords:Trichoderma harzianum, Isozyme and allozyme activities, Protein patterns, Cluster analysis
Subjects:Q Science > QK Botany
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:5679
Deposited By:IR Admin
Deposited On:09 Jan 2013 08:18
Last Modified:18 Feb 2015 12:04

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