Blood meal analysis of tabanid fly after it biting the rare Sumatran rhinoceros

Jeffrine Japning Rovie-Ryan, and Zainal Zahari Zainuddin, and Wahap Marni, and Abdul Hamid Ahmad, and Laurentius N. Ambu, and Junaidi Payne, (2013) Blood meal analysis of tabanid fly after it biting the rare Sumatran rhinoceros. Asian Pacific Journal of Tropical Biomedicine, 3 (2). pp. 95-99.

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Official URL: http://dx.doi.org/10.1016/j.proeng.2013.03.063

Abstract

Objective To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid fly. Methods Blood meal was recovered from the abdomen of an engorged tabanid fly (Haematopota sp.) which was captured immediately after biting a Sumatran rhino in captivity. The blood was applied on to a Whatman FTA® blood card. Subsequent laboratory work was conducted to extract, amplify and sequence the DNA from the sample. Validation was done by sampling the hair follicles and blood samples from the rhinoceros and subjecting it to the same laboratory process. Results BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from the rhino. Conclusions This method could be used in the field application to noninvasively collect genetic samples. Collection of tabanids and other haematophagous arthropods (e.g. mosquitoes and ticks) and other blood-sucking parasites (e.g. leeches and worms) could also provide information on vector-borne diseases. and ticks ) and other blood-sucking parasites ( e.g. leeches and worms ) could also provide information on vector-borne diseases. Journal or Publication Title: Asian Pacific Journal of Tropical Biomedicine

Item Type:Article
Uncontrolled Keywords:Noninvasive DNA sampling, Blood meal,Tabanid fly, Sumatran rhino
Subjects:Q Science > Q Science (General)
Q Science > QL Zoology
ID Code:6130
Deposited By:IR Admin
Deposited On:13 Jun 2013 09:58
Last Modified:17 Feb 2015 15:56

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