Assessment of total phenolic content and free radical-scavenging capacity of Leucaena leucocephala extract

Dorothy Sarim, (2007) Assessment of total phenolic content and free radical-scavenging capacity of Leucaena leucocephala extract. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Plants contain a variety of substances called the phytochemicals, for example phenolic compound and antioxidants. Interest in phenolic antioxidant in the last decade has increased remarkably because of their elevated capacity in scavenging free radical associated with various diseases. In the present study, aqueous, ethanol, and methanol extraction of different parts of Leucaena leucocephala (leaf, stem, and seed) were assessed for their total phenolic content and antioxidant activities. The total phenolic content was measured using Folin-Ciocalteu method while the free radical capacity was evaluated using DPPH (2,2' -diphenyl-l-pierythydrazyl) free-radical-scavenging assay. There are significant differences of total phenolic content in different parts of Leucaena leucocephala. The aqueous extraction of seed shows the highest result in total phenolic content (1.58 mg/ml) while the lowest were found in methanol extraction of stem (0.37mg/ml). The aqueous extraction was considerably better than the ethanol and methanol extraction in all three different part of Leucaena leucocephala. All different extraction for different parts of Leucaena leucocephala can act as radical-scavenger and there were significant differences of antioxidant activities in this different parts of Leucaena leucocepha/a. The highest scavenging effect was found in aqueous extraction of seed (87%) while the lowest scavenging effect were found in ethanol extraction of seed (55%). The results from this study can be used as future references to another study such as preventive effect of Leucaena leucocephala on diseases such as diabetes.

Item Type: Academic Exercise
Uncontrolled Keywords: antioxidants, phenolic compound, Leucaena leucocephala, Folin-Ciocalteu method, free radical capacity, extraction of seed, diabetes
Subjects: Q Science > QD Chemistry
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 16 Jul 2013 05:30
Last Modified: 23 Oct 2017 07:11
URI: http://eprints.ums.edu.my/id/eprint/6569

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