Adsorption characteristics of protein on zeolite adsorbent

Noor Maizura Ismail, (2005) Adsorption characteristics of protein on zeolite adsorbent. Masters thesis, Universiti Teknologi Malaysia.

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Abstract

Adsorption of proteins using zeolites has a great potential in the isolation step of protein industry. Over the years, many interests have been shown in the development of new adsorbents thus has helped the growth of protein separation using adsorption technique. In this research, the protein adsorption capacity of several zeolites is studied over a range of solution pHs. The adsorption of proteins, cytochrome c, α-chymotrypsin and bovine serum albumin on zeolites with various pore diameters, and pH was studied. The concentration of protein was measured using UV/VIS Spectrophotometer. The adsorption capacity for cytochrome c and α chymotrypsin was found to be the highest at pH 9 and 6 respectively. Bovine serum albumin adsorption was highest at pH 3. Increase in pH above the pI value lead to the decrease in the adsorption capacity for a-chymotrypsin and bovine serum albumin. This is postulated to be due to electrostatic repulsion between proteins and the surface of zeolite. The adsorption of different proteins onto H-Beta zeolite, MCM-41, and ZSM-5 shows the effect of protein properties and zeolites to the adsorption capacity. The adsorption isotherm data of protein is approaching the Langmuir model. FTIR analysis was performed for the adsorbed protein to study the interaction between the protein and H-Beta zeolite surface. The observed decrease in the intensities of amide groups in protein structure is most likely a consequence of binding of protein onto H-Beta zeolite.

Item Type:Thesis (Masters)
Uncontrolled Keywords:Adsorption of protein, H-Beta zeolite, isolation, protein structure
Subjects:T Technology > TP Chemical technology
ID Code:6995
Deposited By:IR Admin
Deposited On:18 Sep 2013 11:55
Last Modified:18 Sep 2013 11:55

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