Random Amplified Polymorphic DNA (RAPD) Fingerprinting Of Marine Harmful Algae, Genus Pseudo-Niizschia

Joanna W. Doinsing, (2005) Random Amplified Polymorphic DNA (RAPD) Fingerprinting Of Marine Harmful Algae, Genus Pseudo-Niizschia. Universiti Malaysia Sabah. (Unpublished)

[img]
Preview
Text
ae0000001525.pdf

Download (1MB) | Preview

Abstract

In this study, the Random Amplified Polymorphic DNA (RAPD) technique was used to distinguish three algae species of the genus Pseudo-nitzschia; PNA, PNB and PNC. Genomic DNAs of the three species were first extracted by using modified method of phenol extraction and ethanol precipitation. Of the 16 random primers tested, only 14 amplified DNA segments (OPA-1, OPA-3-5, OPA-7-1O, OPA-12, OPA-14, OPA-16 and OPA-18-20.). From the selected 14 primers, OPA-16 and OPA-18 primers were used for genetic similarity and genetic distance analysis as the bands produced were clear, distinct and reproducible. Genetic similarity and genetic distance of the three species of Pseudo-nitzschia was then calculated by using the Nei and Li (1979) equation. The genetic similarity observed between PNA and PNB was 0.5941, followed by PNB and PNC with value 0.5012 and finally PNA and PNC with value 0.4143 while the genetic distance calculated between PNA and PNB was 0.4059, followed by PNB and PNC with value 0.4988 and finally PNA and PNC with value 0.5857. Cluster analysis of distance values was conducted to construct a dendrogram. From the analysis, it was found that the Pseudo-nitzschia species; PNA was very much closer to PNB compared to PNC.

Item Type: Academic Exercise
Uncontrolled Keywords: Random Amplified Polymorphic DNA, algae, Pseudo-nitzschia, phenol extraction, ethanol precipitation
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 21 Nov 2013 07:48
Last Modified: 17 Oct 2017 05:23
URI: http://eprints.ums.edu.my/id/eprint/7478

Actions (login required)

View Item View Item

Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository