Development of 11 polymorphic microsatellite markers for Xylocarpus granatum(Meliaceae) using next-generation sequencing technology

Yuki Tomizawa, and Yoshimi Shinmura, and Alison K. S. Wee, and Koji Takayama, and Takeshi Asakawa, and Orlex Baylen Yllano, and Severino Garengo Salmo III, and Erwin Riyanto Ardli, and Nguyen, Xuan Tung and Norhaslinda Malekal, and Onrizal, and Sankararamasubramanian Halasya Meenak, and Sarawood Sungkaew, and Mohd Nazre Saleh, and Bayu Adjie, and Khin, Khin Soea and Emiko Oguri, and Noriaki Murakami, and Yasuyuki Watano, and Shigeyuki Baba, and Edward L. Webb, and Tadashi Kajita, (2013) Development of 11 polymorphic microsatellite markers for Xylocarpus granatum(Meliaceae) using next-generation sequencing technology. Conservation Genet Resour, 5. pp. 1159-1162.

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Abstract

Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures.Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum(Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. Nonull allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.

Item Type: Article
Uncontrolled Keywords: Conservation, Genetic diversity , Mangrove , Pyrosequencing , SSR , Indo-West Pacific
Subjects: Q Science > QL Zoology
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 09 Dec 2013 02:14
Last Modified: 11 Oct 2017 07:33
URI: http://eprints.ums.edu.my/id/eprint/7745

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