Screening of phytochemicals and biological activity (antifungal, antibacterial, and anticancer) of medicinal plants

Nur Khairunnisa Ibrahim, (2007) Screening of phytochemicals and biological activity (antifungal, antibacterial, and anticancer) of medicinal plants. Universiti Malaysia Sabah. (Unpublished)

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Abstract

This project was conducted to study the presence of phytochemicals (alkaloid, saponin, and anthraquinone) and the biological activity (antifungal, anticabterial, and PPI inhibitor) of plant samples. A total of nine samples were collected from Orchid De Villa located in Inanam, and one sample was collected around University Malaysia Sabah Campus. All 10 samples were extracted using acetone, methanol and chloroform solvent. Dragendroff's reagent and Wagner's reagent were the two indicator used to test the presence of alkaloid. The result obtained showed that all samples have alkaloid constituents. Foam test and Liebermann-Burchard (LB) test were conducted to testify presence of saponin in all of the samples. The results obtained showed that all samples have negative result for foam test and only Terminalia catappa showed positive result for LB test. For anthraquinone test, no sample showed positive result for both free anthraquinone and C-glycoside bounded anthraquinone. Antifungal screening system involves only Candida albicans. Samples that showed inhibition zone were Mimosa pudica (2.0 cm), Elephantopus tomentosus (2.4 cm), Dendrobium crumenatum (1.7 cm), Opuntia dillenii (1.5 cm), Terminalia catappa (1.6 cm), and Curcuma longa (2.0 cm). Antibacterial screening involves screening against Escherichia coli, Bacillus subtilis, and Salmonella typhi. Extract of Opuntia dillenii (1.3 cm), Terminalia catappa (1.4 cm), and Phyllanthus urinaria (1.3 cm) were testified to have anti-Escherichia coli activity. As for Bacillus subtilis screening, all 10 samples showed positive result. In Salmonella typhi test, all samples showed inhibition except for Elephantopus tomentosus and Curcuma fonga extracts. For PPI inhibitor screening, Terminalia calappa, Polygenum chinense, and Phyllanthus urinaria inhibit both wild (PAY 704-11 H10.018) and mutant (PAY 700-1/ H10.017) strain of yeast cancer cell. Orthosiphon aristatus, Curcuma fonga and Phyllanthus urinaria are also testified to have inhibiiton. However, samples that showed inhibition were testified to be toxic towards both wild and mutant strains. Therefore, none of the samples are potential PPI inhibitor.

Item Type: Academic Exercise
Uncontrolled Keywords: phytochemical, biological activity, DragendrofI's reagent, Wagner's reagent, Liebermann-Burchard (LB) test, inhibitor
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 29 Jan 2014 03:05
Last Modified: 11 Oct 2017 04:50
URI: http://eprints.ums.edu.my/id/eprint/8113

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