Screening of pea (Pisum sativum L.) resistant to powdery mildew disease for Kundasang Highland in Sabah

Evenni Poili, (2009) Screening of pea (Pisum sativum L.) resistant to powdery mildew disease for Kundasang Highland in Sabah. Masters thesis, Universiti Malaysia Sabah.

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Abstract

Pea (Pisum sativum L.) cultivation in Sabah is affected by powdery mildew caused by Erysiphe pisi DC. Fungicide application is the most preferred method of disease control by the growers. However, the non-judicious use of fungicides has led to excessive pesticide residues in the vegetable. Therefore planting of resistant variety is the best option because it is safe, cost effective and practical. Screening of the powdery mildew resistant pea variety was carried out in this study. A total of seven pea varieties were assessed for their response to powdery mildew infection at Agriculture Research Station Mesilou, Kundasang, Ranau. Their plant characters and field performances were also evaluated. Variety S7 was found to be resistant with 0 DSI score in all of the three trials conducted, the rest of the varieties were susceptible with DSI scores of 4.17 - 5.90. Marker linked to the resistance to powdery mildew was identified at about 600bp using PCR-RAPD primer 5'-ATT AGT AGT TGT TGT TG-3'. This band was found in resistant variety (S7) but absent in the susceptible varieties. The powdery mildew resistant pea identified in this study not only has the potential use in the production of resistant cultivars, but the resistant gene once identified and further characterised, can be utilised in the improvement of leguminous plants.

Item Type:Thesis (Masters)
Uncontrolled Keywords:cultivation, pea, powdery mildew disease, fungicide, screening, infection
Subjects:Q Science > QK Botany
Divisions:SCHOOL > School of Science and Technology
ID Code:8714
Deposited By:IR Admin
Deposited On:11 Apr 2014 08:57
Last Modified:11 Apr 2014 08:57

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