Polymorpidsms of cytochrome P450 2E1 (CYP2E1) in gastrointestinal cancer

Keiko Ota Masayoshi, (2008) Polymorpidsms of cytochrome P450 2E1 (CYP2E1) in gastrointestinal cancer. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Gastrointestinal cancer is a cancer that occurred in the digestive system. The cancer includes stomach cancer, liver cancer, pancreas cancer, esophageal cancer, colorectal cancer and anus cancer. This gastrointestinal cancer is known to be associated with the polymorphism of cytochrome P450 2E1 (CYP2E1) gene that are responsible in the oxidation of many low molecular weight procarcinogens and involved in the metabolism of ethanol and acetone. Moreover, the genetic polymorphism of CYP2El gene had been associated to the susceptibility to gastrointestinal cancer. Thus, the purpose of this study is to investigate the polymorphism of CYP2E1 gene and its association with gastrointestinal cancer phenotype. The study was conducted by using the 11 blood samples from the gastrointestinal cancer patients collected from the Queen Elizabeth Hospital. The DNA extraction was done by using the Qiagen Flexigene kit. PCR - RFLP was conducted to determine the genotypes in CYP2E1 using DraI restriction enzyme. The PCR-RFLP was done twice in order to confirm the genotype analysis of CYP2EI polymorphism. As a result, three genotypes were revealed which were the heterozygous (C/D), homozygous variant (C/C) and the homozygous wild type (D/D). From the statistical analysis, allele frequency for D allele (0.56) obtained higher compare to the C allele (0.44). Genotype frequency for heterozygous was 0.4925, homozygous wild type was 0.3136 and homozygous variant was 0.1936. These analysis shows that there was no clear correlation of CYP2EI DraI polymorphism and gastrointestinal cancer.

Item Type: Academic Exercise
Uncontrolled Keywords: gastrointestinal cancer, digestive system, polymorphism of cytochrome, oxidation
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 06 May 2014 06:08
Last Modified: 13 Oct 2017 03:24
URI: http://eprints.ums.edu.my/id/eprint/8858

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