Engineered mycobacterium tuberculosis antigen assembly into core-shell nanobeads for diagnosis of tuberculosis

Nurul Syahidah Sheffee and Patricia Rubio-Reyes and Mayelin Mirabal and Romel Calero and Humberto Carrillo-Calvet and Shuxiong Chen and Chin, Kai Ling and Nur Atiqah Syairah Shakimi and Fadhilah Zulkipli Anis and Siti Suraiya and Maria E. Sarmiento and Mohd Nor Norazmi and Armando Acosta and Bernd H.A. Rehm (2021) Engineered mycobacterium tuberculosis antigen assembly into core-shell nanobeads for diagnosis of tuberculosis. Nanomedicine: Nanotechnology, Biology and Medicine, 34 (1). pp. 1-13. ISSN 1549-9634

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Abstract

Despite recent advances in diagnosis, tuberculosis (TB) remains one of the ten leading causes of death worldwide. Here, we engineered Mycobacterium tuberculosis (Mtb) proteins (ESAT6, CFP10, and MTB7.7) to self-assemble into core-shell nanobeads for enhanced TB diagnosis. Respective purified Mtb antigen-coated polyester beads were characterized and their functionality in TB diagnosis was tested in whole blood cytokine release assays. Sensitivity and specificity were studied in 11 pulmonary TB patients (PTB) and 26 healthy individuals composed of 14 Tuberculin Skin Test negative (TSTn) and 12 TST positive (TSTp). The production of 6 cytokines was determined (IFNγ, IP10, IL2, TNFα, CCL3, and CCL11). To differentiate PTB from healthy individuals (TSTp + TSTn), the best individual cytokines were IL2 and CCL11 (>80% sensitivity and specificity) and the best combination was IP10 + IL2 (>90% sensitivity and specificity). We describe an innovative approach using full-length antigens attached to biopolyester nanobeads enabling sensitive and specific detection of human TB.

Item Type:	Article
Keyword:	Tuberculosis , Mycobacterium Tuberculosis , Biopolyester Beads , Polyhydroxybutyrate , Interferon-Γ Release Assay , Antigen Carrier System
Subjects:	R Medicine > R Medicine (General) R Medicine > RB Pathology
Department:	FACULTY > Faculty of Medicine and Health Sciences
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	18 Jun 2021 08:15
Last Modified:	18 Jun 2021 08:15
URI:	https://eprints.ums.edu.my/id/eprint/27284

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