High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique

Rosmah Murdad and Hwa, Kuik Sok and Seng, Choo Khen and Mariam Abd Latip, Datin and Zaleha Abdul Aziz and Rimi Ripin (2006) High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique. Scientia Horticulturae, 111 (1). pp. 73-79. ISSN 0304-4238


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This study was conducted to determine the effects of coconut water (CW) and activated charcoal (AC) on multiplication of Phalaenopsis gigantea protocorms. The protocorms used for this study were obtained by germinating seeds in vitro. Protocorms with trimmed and untrimmed bases were cultured on XER basal medium containing 0, 10, 15 or 20% (v/v) CW; and 0, 1, 2 or 2.5 g ACl(-1). Trimmed protocorms exhibited the highest percentage of proliferation on a medium containing 15% (v/v) CW and 2.5 g AC F 1 (56.82 +/- 38.86%) with an average of 4.24 +/- 2.89 pro-protocorms formed per protocorm. Untrimmed protocorms cultured on a medium containing 20% (v/v) CW without AC produced the highest percentage of new protocorms (6.93 +/- 6.28%) with an average of 0.72 +/- 0.57 per protocorm. When CW was added to a medium singly, 10% (v/v) CW induced a higher degree of proliferation on trimmed protocorms (5.68 +/- 10.14%) with an average 0.50 +/- 0.84 new protocorms per protocorm. Untrimmed protocorms proliferate to a much lower extent (2.57 +/- 2.74%) with an average of 0.72 +/- 0.57 protocorms per protocorm when cultured on a similar medium. A high concentration of CW enhanced proliferation on untrimmed protocorms, but increased mortality of trimmed protocorms. The addition of CW with AC to media increased protocorm proliferation and survival of both trimmed and untrimmed protocorms. When cultured on all media, trimmed protocorms produced a higher number of new protocorms (an average 0.5-7.0) as compared to untrimmed protocorms (0.3-1.9). Comparative studies showed that trimmed protocorms produced up to 10 times more new protocorms than untrimmed ones. Altogether this study showed that trimmed protocorms cultured on a medium containing CW and AC can be used for high-frequency multiplication of P. gigantea seedlings. (c) 2006 Elsevier B.V. All rights reserved.

Item Type: Article
Keyword: Protocorms, Trimmed and untrimmed, Protocorms, New per protocorm, Phalaenopsis gigantea
Subjects: S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB183-317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants
Department: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 29 Sep 2011 18:46
Last Modified: 19 Oct 2017 09:51
URI: https://eprints.ums.edu.my/id/eprint/1140

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