Determination of nicotine content in various tobacco products using capillary electrophoresis

Romero Robby David Ubing (2010) Determination of nicotine content in various tobacco products using capillary electrophoresis. Universiti Malaysia Sabah. (Unpublished)


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This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer and sodium phosphate, where both buffer was also tested at different pH pH 2.5, 4.5 and 9.5 .Sodium phosphate buffer with pH 2.5 was chosen as a running buffer due to high and symmetrical peak. The buffer then is tested on using different concentration 10mM, 25mM, 50mM and 100mM. Buffer with a high concentration increases the migration time slightly. Based .on the result sodium phosphate buffer pH 2.5 with 100mM was use in the sample analyze using CE and the condition was 10cm capillary length, UV detection at 260 nm, sample injection 0.5 psi, for 5 s at 30ºC with 5 kV applied voltage. Sample was analyze using the optimized method after extraction and the result was Cigarette (12.921 ppm) have almost double the nicotine contain compare to local chewing tobacco (7.572 ppm).

Item Type: Academic Exercise
Keyword: tobacco, separation of nicotine, capillary electrophoresis (CE), citrate buffer, sodium phosphate, extraction
Subjects: T Technology > TP Chemical technology
Department: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 11 May 2016 13:54
Last Modified: 27 Oct 2017 10:16

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