Hossain, Amzad and Zhari Ismail (2012) Quantification and enrichment of sinensetin in the leaves of Orthosiphon stamineus. Arabian Journal of Chemistry. ISSN 1878-5352
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Abstract
Sinensetin was identified in the leaves of Orthosiphon stamineus by thin-layer chromatography, GC coupled with mass spectrometry (MS), UV, IR and 1H-NMR spectroscopy. Enrichment of sinensetin by using different mixture solvent system and the quantification analysis by thin-layer chromatography-imaging densitometric method using as external standard method showed the highest concentration of 0.32% of sinensetin in the mixture solvent of acetone-water (70:30) when compared to methanol-water (1:1) of only 0.15% in the leaves of Orthosiphon stamineus. The TLC densitometer, although yields slightly higher values than the other analytical methods, is preferred due to its simplicity, ease and low cost.
| Item Type: | Article |
|---|---|
| Keyword: | Acetone-water, Densitometer, Enrichment, Mixture solvents, Sinensetin |
| Subjects: | Q Science > QD Chemistry |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 02 Jan 2013 11:17 |
| Last Modified: | 01 Jan 2021 19:00 |
| URI: | https://eprints.ums.edu.my/id/eprint/1236 |
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