Kenneth Francis Rodrigues and Kobun Rovina and Shafiquzzaman Siddiquee and Nor Azah Yusof and Suryani Saallah (2014) Nanoparticle-enhanced electrochemical biosensor with DNA immobilization and hybridization of trichoderma harzianum gene. Sensing and Bio-Sensing Research, 2. pp. 16-22. ISSN 2214-1804
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Abstract
The genus Trichoderma is a soil-borne fungi which in numerous reports has been successfully used as a biological control agent against various plant pathogens. The identification of Trichoderma species worldwide is currently deduced from micro-morphological descriptions which are tedious and prone to error. Electrochemical approaches are currently being developed for the detection and analysis of DNA. In the present study, an electrochemical DNA biosensor was successfully developed based on ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles and a chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). A single-stranded DNA probe was immobilized on this electrode. Methylene blue (MB) was used as the hybridization indicator to monitor the hybridization reaction of the target DNA. Under optimal conditions using differential pulse voltammetry (DPV), the target DNA sequences were detectable at concentration ranges of 1.0 × 10−18–1.82 × 10−4 mol L−1, and the detectable limit was 1.0 × 10−19 mol L−1. The developed DNA biosensor enables the study of hybridization with crude DNA fragments and the results of this study confirm that this DNA biosensor provides a fast, sensitive and convenient way for the species level identification of Trichoderma harzianum.
| Item Type: | Article |
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| Keyword: | DNA electrochemical biosensor, DNA hybridization, ZnO nanoparticles, Ionic liquid, Chitosan, Genus Trichoderma |
| Subjects: | T Technology > TP Chemical technology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 28 Sep 2016 14:38 |
| Last Modified: | 23 Oct 2017 14:58 |
| URI: | https://eprints.ums.edu.my/id/eprint/14526 |
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