SRAP markers based genetic analysis of silene species

Tahereh Aghaee Bargish and Fatemeh Rahmani (2016) SRAP markers based genetic analysis of silene species. Journal of Tropical Biology & Conservation (JTBC), 13. pp. 57-70. ISSN 1832-3902


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Plant genetic resources are an essential part of the world’s natural resources, and knowledge of genetic diversity and its distribution is essential for plant conservation. In the present work, the genetic variation was studied among thirteen species of Silene genus. Samples were collected from different locations of West Azarbijan and Khorasan provinces in Iran. This investigation, for the first time, has studied sequence-related amplified polymorphism (SRAP) marker to assess the genetic diversity and genetic similarity among 13 species of Silene in Iran. These species belonge to eight sections including Sclerocalycinae, Melandriforms, Lychnidiforms, Inflatae, Lasiocalycinae, Spergolifoliae, Auriculatae and Conoimorpha. Fifteen SRAP primer combinations generated 62 fragments, of which 46 (71.90 %) were polymorphic. Percentage of polymorphism ranged from 50 % to a maximum of 100 % and Jaccard’s similarity coefficient ranged from 0.48 to 0.91. Minimum Evolution analysis revealed four main clusters. The study indicates that SRAP markers with targeting ORFs, high reproducibility and optimal marker distribution could be good candidates for assessing genetic variation in Silene.

Item Type: Article
Keyword: Genetic variation, Silene, polymorphism, SRAP
Subjects: S Agriculture > SB Plant culture
Department: INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User: Munira M
Date Deposited: 10 Feb 2017 15:28
Last Modified: 24 Nov 2020 11:10

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