In-ovo injection of tongkat ali (Eurycoma longifolia) extract on the pre-hatch development of chicken embryo

Loh, Chai Yuen (2015) In-ovo injection of tongkat ali (Eurycoma longifolia) extract on the pre-hatch development of chicken embryo. Universiti Malaysia Sabah. (Unpublished)


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This study was conducted to investigate the effects of in-ovo administration of Tongkat Ali (TA) on the mortality and pre-hatch development of chicken embryo. The fertile eggs of cross-bred village chicken were injected with liquid TA that diluted from powder form at the beginning of incubation. Initially, a dosage response study of TA (0 mg, 0.1 mg, 1 mg and 10 mg/ 100g egg) was conducted. Then, the effects of 0.1 mg/ 100g egg TA were investigated on the embryonic mortality and body parts development until 19th day of incubation. Results showed a toxic effect of TA on the embryonic survivability and development, and the LDâ‚…â‚€ was found at around 10 mg/ 100g egg in comparison of control mortality. At 88 to 96 hours of incubation, the 0.1 mg/ 100g egg TA injection (38 somites) significantly reduced the number of somite in the embryo as compared to that in the control (42 somites). At 19th day of incubation, this dosage also attributed an insignificantly lower embryonic weight (53.38 % to egg weight) than in the control (70.50 % to egg weight). The dosage applied showed the insignificant difference on the wing length at 9th, 16th and 19th day of incubation. For limb length parameter, the TA dosage had showed a significant higher in 19th day TA injected chick embryo. Relatively much earlier and huge feather-follicle development was observed in the TA injected embryos compared to control. On the day 19th of incubation, 100 % TA exposed chicks produced a traceable vocal sound along with pipping mark in the shell, but these phenomena were completely absent in the chick of control group. This study suggested that administration of TA through in-ovo injection route could bring both the positive and negative effects in developing chicken embryo, however, more studies are required to elucidate the mechanisms of TA effect including the embryonic sex-specificity in different poultry species.

Item Type: Academic Exercise
Keyword: in-ovo administration, Tongkat Ali, chicken embryo, pre-hatch development
Subjects: S Agriculture > SF Animal culture
Department: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Munira M
Date Deposited: 05 Jul 2017 09:57
Last Modified: 16 Oct 2017 12:05

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