Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode

Shafiquzzaman Siddiquee and Nor Azah Yusof and Abu Bakar Salleh and Tan, Soon Guan and Fatimah Abu Bakar (2012) Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode. Journal of Solid State Electrochemistry, 16. pp. 273-282. ISSN 1432-8488 (In Press)

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URL: https://link.springer.com/article/10.1007/s10008-0...

Abstract

Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0 Ã— 10-18 to 1.82 Ã— 10-4 mol L-1, and with the detection limit of 1.0 Ã— 10-19 mol L-1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum.

Item Type:	Article
Keyword:	DNA electrochemical biosensor, DNA hybridization, ZnO nanopaticles, Ionic liquid, Chitosan
Subjects:	Q Science > Q Science (General) > Q1-390 Science (General) Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	30 Jun 2023 09:19
Last Modified:	30 Jun 2023 09:19
URI:	https://eprints.ums.edu.my/id/eprint/1752

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