Noor Aini Bohari and Shafiquzzaman Siddiquee and Kamaruzaman Hj. Ampon (2016) Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol). Biosensors, 6 (3). p. 32. ISSN 2079-6374
|
Text
Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples.pdf Download (48kB) | Preview |
Abstract
Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD+ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique.
| Item Type: | Article |
|---|---|
| Keyword: | formaldehyde biosensor, glassy carbon electrode, gold nanoparticles, ionic liquid, methylene blue |
| Subjects: | T Technology > TP Chemical technology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | MUNIRA BINTI MARASAN - |
| Date Deposited: | 13 Mar 2018 11:24 |
| Last Modified: | 13 Mar 2018 11:24 |
| URI: | https://eprints.ums.edu.my/id/eprint/19364 |
Actions (login required)
 |
View Item |