Clarence M. Ongkudon and Michael K. Danquah (2011) Endotoxin removal and plasmid DNA recovery in various metal ion solutions. Separation Science and Technology, 46 (8). pp. 1280-1282. ISSN 1520-5754
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Abstract
In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (∼91%) as well as high plasmid recovery (∼100%) compared to other metal ions tested.
| Item Type: | Article |
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| Keyword: | free metal ion, lipopolysacharide, plasmid DNA, selective precipitation |
| Subjects: | Q Science > QP Physiology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | MUNIRA BINTI MARASAN - |
| Date Deposited: | 27 Mar 2018 15:25 |
| Last Modified: | 27 Mar 2018 15:25 |
| URI: | https://eprints.ums.edu.my/id/eprint/19592 |
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