Improvements in separation of peptides using capillary electrophoresis

Mohd Nazri Hafiz Mohd Khairi (2008) Improvements in separation of peptides using capillary electrophoresis. Universiti Malaysia Sabah. (Unpublished)

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In this review, various aspect concerning the resolution of capillary electrophoresis for nine standard peptides ( namely, bradykinin, bradykinin fragment 1-5, substance P, Arg-vasopressin, luteinizing hormone┬Ěreleasing hormone (LHRH), bombesin, leucine-enkephalin, methionine-enkephalin and oxytocin). The experiment were carried out using 100 mM phosphate buffer as the separation buffer, added with acetonitrile (ACN) and triethylamine (TEA) as an additive at low pH. The resolution of these peptides was examined at different concentrations of ACN ( 0, 10, 20, 30, 40, 50 and 60 %) and TEA ( 0, 5, 10, 20, 30, 40 ppm) at their respective final pH. The results showed that the resolution was improve when 10 % of ACN added in the BGE. The optimum resolution found at 40 % ACN where all peptides were resolved at baseline. The shape was perfectly symmetrical. As for the TEA addition, adding 5 ppm already improve the peaks and addition of 30 ppm TEA gives the optimum resolution compare to all. For both ACN and TEA, adding more concentrated additive gives poor resolution of peptides. The investigation was continued by applying voltage ( 23, 24, 25,26 and 27 kV). Shorter time separation achieved when voltage increased. The best resolution found at 25 kV. However, more voltage caused poor resolution.

Item Type: Academic Exercise
Keyword: capillary electrophoresis, peptide, voltage
Subjects: Q Science > QD Chemistry
Department: FACULTY > Faculty of Science and Natural Resources
Date Deposited: 09 Apr 2018 09:34
Last Modified: 09 Apr 2018 09:34

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