In vitro culture of Borneo's endemic orchid, vanda dearei

Roslina Jawan and Jualang Azlan Gansau and Ong, Janna Abdullah (2010) In vitro culture of Borneo's endemic orchid, vanda dearei. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18 (1). pp. 201-205. ISSN 0128-7451


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The effect of basal media, complex additives and sugars to the growth and development of V. dearei protocorms were reported in this study. Protocorms were initiated from in vitro seeds germination from immature capsule. Among the three basal media, growth of protocorms was superior in 1/2 MS medium followed by KC and VW with maximum percentage of protocorms with leaf and root, and mean number of leaf and root produced. Meanwhile, 0.2% (w/v) yeast extract markedly enhanced the growth of protocorms. In sugar study, results show that protocorms preferred sucrose as compared to fructose and glucose. The leaf and root formation was significantly enhanced as the sugar concentration was decreased. Medium supplemented with 2% (w/v) sucrose was the best compared to the other treatments and sugar at a concentration of 4% (w/v) induced the formation of large size seedlings.

Item Type: Article
Keyword: Growth medium, Propagation, Protocorms, Vanda dearei
Subjects: Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms
Department: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 10 Mar 2011 18:24
Last Modified: 13 Oct 2017 10:09

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