Mariam Abd Latip, Datin and Rosmah Murdad and Zaleha Abdul Aziz and Ting, Lai Hui and Lilly Malar, Govindasamy and Rimi Ripin (2010) Effects of N6-benzyladenine and thidiazuron on proliferation of phalaenopsis gigantea protocorms. Asia-Pacific Journal of Molecular Biology and Biotechnology, Volume (1). pp. 215-218. ISSN 0128-7451
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Abstract
Cytokinins are known to promote multiplication and development of protocorms in orchid plants. Potential effects of N6-benzyladenine (BA) and thidiazuron (TDZ) on the proliferation of Phalaenopsis gigantea protocorms were investigated. The seed-derived protocorm sections were cultured on solidified NDM (New Dogashima Medium) basal medium containing 0-5.0 mg l-1 BA; or 0-1.0 mg l-1 TDZ. Sections cultured on the media containing low concentrations of BA (0.5 to 3.5 mg l-1) or TDZ (0.1 to 1.0 mg l-1) multiplied faster as compared to those cultured on other media with higher concentrations of BA and the control medium. Approximately 28% of the sections proliferated after 40 days in culture. The percentage of protocorm sections proliferate increased significantly up to 58 -66% (with 1-20 new protocorms per section) on media containing TDZ at the concentrations of 0.1 - 0.3 mg l-1 after 80 days of culture. In the presence of BA, however, the percentage of sections produced new protocorms was significantly lower, ranging from 5 to 30% with 1-46 new protocorms formed per section.
| Item Type: | Article |
|---|---|
| Keyword: | Orchid, Phalaenopsis gigantea, Propagation, Protocorms |
| Subjects: | Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms |
| Department: | SCHOOL > School of Sustainable Agriculture |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 10 Mar 2011 18:32 |
| Last Modified: | 13 Oct 2017 10:11 |
| URI: | https://eprints.ums.edu.my/id/eprint/2169 |
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