Effects of N6-benzyladenine and thidiazuron on proliferation of phalaenopsis gigantea protocorms

Mariam Abd Latip, Datin and Rosmah Murdad and Zaleha Abdul Aziz and Ting, Lai Hui and Lilly Malar, Govindasamy and Rimi Ripin (2010) Effects of N6-benzyladenine and thidiazuron on proliferation of phalaenopsis gigantea protocorms. Asia-Pacific Journal of Molecular Biology and Biotechnology, Volume (1). pp. 215-218. ISSN 0128-7451


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Cytokinins are known to promote multiplication and development of protocorms in orchid plants. Potential effects of N6-benzyladenine (BA) and thidiazuron (TDZ) on the proliferation of Phalaenopsis gigantea protocorms were investigated. The seed-derived protocorm sections were cultured on solidified NDM (New Dogashima Medium) basal medium containing 0-5.0 mg l-1 BA; or 0-1.0 mg l-1 TDZ. Sections cultured on the media containing low concentrations of BA (0.5 to 3.5 mg l-1) or TDZ (0.1 to 1.0 mg l-1) multiplied faster as compared to those cultured on other media with higher concentrations of BA and the control medium. Approximately 28% of the sections proliferated after 40 days in culture. The percentage of protocorm sections proliferate increased significantly up to 58 -66% (with 1-20 new protocorms per section) on media containing TDZ at the concentrations of 0.1 - 0.3 mg l-1 after 80 days of culture. In the presence of BA, however, the percentage of sections produced new protocorms was significantly lower, ranging from 5 to 30% with 1-46 new protocorms formed per section.

Item Type: Article
Keyword: Orchid, Phalaenopsis gigantea, Propagation, Protocorms
Subjects: Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms
Department: SCHOOL > School of Sustainable Agriculture
Depositing User: ADMIN ADMIN
Date Deposited: 10 Mar 2011 18:32
Last Modified: 13 Oct 2017 10:11
URI: https://eprints.ums.edu.my/id/eprint/2169

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