Eugene Marfo Obeng and Clarence M. Ongkudon and Cahyo Budiman and Ruth Maas and Joachim Jose (2018) An optimal blend of single auto displayed cellulases for cellulose saccharification – a proof of concept. Journal of Chemical Technology & Biotechnology. pp. 1-10.
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Abstract
BACKGOUND: To date, it has been challenging to determine the optimal proportions of the individual cellulases in crude extracts and multienzyme systems to promote enzyme synergism. The application of single surface-display of enzymes may obviate such challenges. This article presents a proof of concept of the optimal mixing of single surface-displayed cellulases for cellulose hydrolysis using Simplex lattice mixture design. RESULTS: The recently discovered maximized autotransporter-mediated expression system was used to express the three cellulases. The biochemical screening analysis revealed that the enzymes have a broad range of functional pH (4–9) and temperature (30–100 ∘C) characteristics. The evaluated optima were pH 6 and 60 ∘C. Through the statistical design of experiment, a blend ratio of 1: 1.6: 1.4 of endoglucanase: exoglucanase:
| Item Type: | Article |
|---|---|
| Keyword: | cellulases, synergism, autodisplay technology, bacterial surface display, enzyme blend, mixture design |
| Subjects: | T Technology > T Technology (General) T Technology > TP Chemical technology |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | SITI AZIZAH BINTI IDRIS - |
| Date Deposited: | 26 Nov 2019 14:15 |
| Last Modified: | 26 Nov 2019 14:15 |
| URI: | https://eprints.ums.edu.my/id/eprint/24196 |
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