In vitro and in vivo effects of Puntius javanicus cholinesterase by copper

Mohd Khalizan Sabullah and Mohd Rosni Sulaiman and Mohd Yunus Abd Shukor and Nor Aripin Shamaan and Ariff Khalid and Siti Aqlima Ahmad (2015) In vitro and in vivo effects of Puntius javanicus cholinesterase by copper. Fresenius Environmental Bulletin, 24 (12b). pp. 4615-4621. ISSN 1018-4619

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Abstract

The aim of this study is to determine the inhibitory effect of copper towards butyrylcholinesterase (BChE) activity. Using the Lineweaver-Burk plot, Puntius javanicus BChE activity was found to be noncompetitively inhibited by copper. The maximal velocities of untreated (control) BChE, 0.5 and 10 mg/L copper-treated BChE are 53.70, 31.81 and 14.30 Umg-1, respectively, while the biomolecular constant (Km) values of both tests shows no significant difference (p>0.05). The in vitro IC50 of copper ion to the BChE was found to be 0.0948 (0.0658 to 0.1691) mg/L. In vivo tests showed that in the presence of 0.1 mg/L copper, the BChE activity was slightly higher compared to the untreated control. Copper sulfate at 0.3 mg/L concentration showed no significant inhibition compared to control. However, the activity decreased with increasing copper concentrations of 0.5, 1.0 and 5.0 mg/L, with the remaining activity at 87.60, 84.60 and 73.00 %, respectively. This study suggests that BChE isolated from P. javanicus liver tissue is a potentially new source of biomarker for copper contamination.

Item Type: Article
Keyword: Butyrylcholinesterase (BChE) , P. javanicus , Copper
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301-705.5 Biology (General)
S Agriculture > SH Aquaculture. Fisheries. Angling
Department: FACULTY > Faculty of Science and Natural Resources
Depositing User: SAFRUDIN BIN DARUN -
Date Deposited: 29 Jun 2021 07:29
Last Modified: 29 Jun 2021 07:29
URI: https://eprints.ums.edu.my/id/eprint/27470

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