Antiparasitic potential of chromatographic fractions of nephrolepis biserrata and liquid chromatography-quadrupole time-of-flight-mass spectrometry analysis

Muhammad Dawood Shah and Kazuki Tani and Yoong, Soon Yong and Fui, Fui Ching and Shaleh, S.R.M. and Vairappan, C.S. and Venmathi Maran (2021) Antiparasitic potential of chromatographic fractions of nephrolepis biserrata and liquid chromatography-quadrupole time-of-flight-mass spectrometry analysis. Molecules, 26. pp. 1-12. ISSN 1420-3049

	Text Antiparasitic potential of chromatographic fractions of nephrolepis biserrata and liquid chromatography-quadrupole time-of-flight-mass spectrometry analysis.pdf Download (43kB)
	Text Antiparasitic_Potential_of_Chromatographic_Fractio.pdf Restricted to Registered users only Download (2MB)

URL: https://www.scopus.com/inward/record.uri?partnerID...

Abstract

Marine aquaculture development is recently impeded by parasitic leech Zeylanicobdella arugamensis (Hirudinea, Piscicolidae) in Sabah, Malaysia. The parasitic leech infests a variety of cultured fishes in aquaculture facilities. In this study, we evaluated the antiparasitic activity of the chromatographic fractions of the medicinal plant Nephrolepis biserrata methanol extract against Z. arugamensis and highlighted the potential metabolites responsible for the antiparasitic properties through liquid chromatography (LC)–quadrupole time-of-flight (QTOF)–mass spectrometry (MS) analysis. Out of seven fractions obtained through flash column chromatography techniques, three fractions demonstrated antiparasitic properties. Significant parasitic mortality was indicated by fraction 3 at a concentration of 2.50 mg/mL, all the leeches were killed in a time limit of 1.92 ± 0.59 min. followed by fraction 4 (14 mg/mL) in 34.57 ± 3.39 and fraction 5 (15.3 mg/mL) in 36.82 ± 4.53 min. LC-QTOF-MS analysis indicated the presence of secondary metabolites including phytosphingosine (6), pyrethrosin (1), haplophytine (9), ivalin (2), warburganal (3), isodomedin (4) and pheophorbide a (16), representing sphingoid, alkaloid, terpenoid, phenolic and flavonoid groups. Thus, our study indicated that the chromatographic fractions of N. biserrata demonstrated significant antiparasitic activity against the marine parasitic leeches due to the presence of potent antiparasitic bioactive compounds.

Item Type:	Article
Keyword:	Natural products , Aquaculture , Grouper , Zeylanicobdella , Antiparasitic , Secondary metabolites , Leeches , LC-QTOF-MS
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Department:	INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	28 Sep 2021 14:43
Last Modified:	28 Sep 2021 15:23
URI:	https://eprints.ums.edu.my/id/eprint/29182

Actions (login required)

View Item