Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples

Yuszniahyati Yaakob and Kenneth Francis Rodrigues and Fernandes Opook and Timothy William and Daisy Vanitha John (2017) Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples. Malaysian Journal of Medical Science, 25. pp. 44-51. ISSN 1394-195X (P-ISSN) , 2180-4303 (E-ISSN)

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URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC57728...

Abstract

Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk. Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-based ELISA was compared with a microscopic agglutination test (MAT), which is currently used as the gold standard for the diagnosis of leptospirosis. Results: Our results showed that all the MAT-positive serum samples were positive for Leptospira-specific IgG in an ELISA, while 65% (n = 13) of these samples were also positive for Leptospira-specific IgM. In the MAT-negative serum samples, 80% and 55% of the samples were detected as negative by an ELISA for Leptospira-specific IgM and IgG, respectively. Conclusion: An ELISA using the synthetic rLipL32 antigen was able to distinguish Leptospira-specific IgM (sensitivity 65% and specificity 80%) and IgG (sensitivity 100% and specificity 55%) in human serum samples and has the potential to serve as a rapid diagnostic test for leptospirosis.

Item Type:	Article
Keyword:	Leptospirosis , LipL32 protein , Enzyme-linked immunosorbent assay , Codon optimised , Anti-leptospira antibodies
Subjects:	R Medicine > RB Pathology > RB1-214 Pathology > RB37-56.5 Clinical pathology. Laboratory technique
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	21 Aug 2021 12:09
Last Modified:	21 Aug 2021 12:09
URI:	https://eprints.ums.edu.my/id/eprint/30245

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