In vitro seed germination of Coelogyne asperata Lindl. (Orchidaceae)

Arnnyitte Alexander and Jualang Azlan Gansau and Devina David (2021) In vitro seed germination of Coelogyne asperata Lindl. (Orchidaceae). Transactions on Science and Technology, 8. pp. 1-6. ISSN 2289-8786

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Abstract

A protocol for in vitro seed germination of Coelogyne asperata Lindl. has been established successfully. Immature seeds from 182 days old capsule were cultured on three different basal media; Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) and maintained under continuous light at 25 ± 2 ˚C. After 30 days of culture (DAC), more than 90% of seeds were germinated on KC and VW media and about 84% of seeds germinated on MS medium. The incorporation of organic additives, including coconut water, potato homogenate and tomato juice each at 10% (v/v) in KC medium was tested to determine their effect on seed germination of C. asperata. The result revealed that KC basal medium alone without addition of organic additives promoted over 90% of seed germination at 30 DAC. Therefore, the protocol of using standard KC basal medium for C. asperata seeds germination could be suggested for mass propagation and conservation of this wild scented orchid.

Item Type:	Article
Keyword:	Orchidaceae , Asymbiotic seed germination , Knudson C , Organic additives
Subjects:	S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB403-450.87 Flowers and flower culture. Ornamental plants > SB409-413 Culture of individual plants
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	20 Sep 2021 15:43
Last Modified:	20 Sep 2021 15:43
URI:	https://eprints.ums.edu.my/id/eprint/30444

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