Myo Thura Zaw and Nor Amalina Emran and Mohd Yusof Ibrahim and Maria Suleiman and Tajul Ariffin Awang Mohd and Aza Sherin Yusuff and Khin Saw Naing and Than Myint and Muhammad Jikal and Mohd Azmi Salleh and Zaw Lin (2019) Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015. Journal of Microbiology, Immunology and Infection, 52. pp. 563-570. ISSN 1684-1182 (P-ISSN) , 1995-9133 (E-ISSN)
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Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 ABSTRACT.pdf Download (66kB) |
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Abstract
Background: Cholera is an important health problem in Sabah, a Malaysian state in northern Borneo; however, Vibrio cholera in Sabah have never been characterized. Since2002, serogroup O1 strains having the traits of both classical and El Tor biotype, designated as atypical El Tor biotype, have been increasingly reported as the cause of cholera worldwide. These variants are believed to produce clinically more severe disease like classical strains. Purpose: The purpose of this study is to investigate the genetic diversity of V. cholera in Sa-bah and whether V. cholera in Sabah belong to a typical El Tor biotype. Methods: ERIC-PCR, a DNA fingerprinting method for bacterial pathogens based on the entero-bacterial repetitive intergenic consensus sequence, was used to study the genetic diversity of65 clinical V. choleraeO1 isolates from 3 districts (Kudat, Beluran, Sandakan) in Sabah and one environmental isolate from coastal sea water in Kudat district. In addition, we studied the biotype-specific genetic traits in these isolates to establish their biotype. Results: Different fingerprint patterns were seen in isolates from these three districts but one of the patterns was seen in more than one district. Clinical isolates and environmental isolate have different patterns. In addition, Sabah isolates harbor genetic traits specific to both classical biotype (ctxB-1,rstRCla) and El Tor biotype (rstRET,rstC,tcpAET,rtxC, VC2346). Conclusion: This study revealed that V. cholera in Sabah were genetically diverse and were atypical El Tor strains. Fingerprint patterns of these isolates will be useful in tracing the origin of this pathogen in the future.
| Item Type: | Article |
|---|---|
| Keyword: | Cholera , Vibrio cholerae , Genetic diversity , Atypical El Tor , Sabah |
| Subjects: | R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC109-216 Infectious and parasitic diseases |
| Department: | FACULTY > Faculty of Medicine and Health Sciences |
| Depositing User: | SAFRUDIN BIN DARUN - |
| Date Deposited: | 22 Oct 2021 11:03 |
| Last Modified: | 22 Oct 2021 11:03 |
| URI: | https://eprints.ums.edu.my/id/eprint/30570 |
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