Inoculum sizes of locally isolated phototrophic bacterium on the utilization of palm mill effluent

Sujjat Al Azad and Sitti Raehanah Muhammad Shaleh (2015) Inoculum sizes of locally isolated phototrophic bacterium on the utilization of palm mill effluent. British Biotechnology Journal, 8. pp. 1-11. ISSN 2231-2927

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Abstract

To produce Rhodobacter sphaeroides strain UMSPSB3 biomass with the reduction of chemical oxygen demand (COD) from palm oil mill effluent. Locally isolated phototrophic bacterium with different inoculum levels were used in Palm Oil Mill effluent (POME). Collected POME was characterized before used as substrate. Inoculum of bacterium was grown in synthetic media and 48 hours inoculum was used to utilize the substrate. Biotechnological laboratory, Borneo Marine Research Institute, University Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia, between February 2014 to April 2014. Growth characteristics of bacterium Rhodobacter sphaeroides strain UMSPSB3 was monitored at different light intensities. Later phototrophic bacterium Rhodobacter sphaeroides strain UMSPSB3 was grown in settled non-sterilized Palm Oil Mill effluent (POME). The growth characteristics of bacterium in term of dry cell weight and total carotenoids production, and reduction of COD were compared using 10%, 20% and 30% (v/v) levels of inoculum developed in synthetic 112 media.

Item Type: Article
Keyword: Phototroph , Inoculum size , Palm oil mill effluent , Growth , Bioremediation
Subjects: Q Science > QR Microbiology > QR1-502 Microbiology > QR75-99.5 Bacteria
T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP248.13-248.65 Biotechnology
Department: INSTITUTE > Borneo Marine Research Institute
Depositing User: DG MASNIAH AHMAD -
Date Deposited: 26 Oct 2021 12:57
Last Modified: 26 Oct 2021 12:57
URI: https://eprints.ums.edu.my/id/eprint/30694

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