Nadia Iryani Najri and Vijay Kumar Subbiah and Noor Hydayaty Md. Yusuf and Rashidah Mohammad and Mohammad Zahirul Hoque (2021) Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype. In: 14th Malaysia International Genetic Congress (MIGC14), 15 -17 March 2021, Malaysia (Virtual Conference).
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Abstract
Introduction: MicroRNAs (miRNAs) are highly promising as biomarkers and are an attractive tool for novel therapeutic approaches. The expression of miRNAs in patient’s serum has been broadly used as biomarker candidates against viral infection. Circulatory miRNAs can directly regulate viral genes either by promoting or repressing viral replications. Objectives: Thus, we attempted to identify serotype specific miRNA in patients with dengue infection. For the purpose of this paper, we present the expression profile of microRNAs in Dengue Serotype 1 (DENV-1) patients. Methodology: A total of 40 patients with a single DENV-1 serotype infection were identified along with 40 healthy controls. Serum RNAs was isolated from these subjects and subjected to high-throughput small RNA (sRNA) sequencing. This research was approved by the Medical Research Ethics Committee (MREC), Ministry of Health, Malaysia (No. NMRR-18-2782-42195). Result: After, trimming and quality control of the reads, we shortlisted 35 miRNAs candidates that were promising for downstream analysis. From this, 24 miRNAs was upregulated in DENV-1 while, 11 was downregulated in the sera of patients. Conclusion: Differential expression of microRNA may serve as reliable biomarkers of disease severity during early stages of dengue infection.
| Item Type: | Conference or Workshop Item (Paper) |
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| Keyword: | MicroRNA , Dengue patients , Denv-1 serotype |
| Subjects: | R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA421-790.95 Public health. Hygiene. Preventive medicine > RA643-645 Disease (Communicable and noninfectious) and public health |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | SAFRUDIN BIN DARUN - |
| Date Deposited: | 22 Oct 2021 09:37 |
| Last Modified: | 22 Oct 2021 09:37 |
| URI: | https://eprints.ums.edu.my/id/eprint/31000 |
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