Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong)

Hoo, Kah Yan and Lee, Ping Chin and Mariam A. Latip and Loumi @ Noumie Surugau and Zaleha A. Aziz (2021) Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong). Jurnal Teknologi (Sciences and Engineering), 83. pp. 91-100. ISSN 2180-3722

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URL: https://journals.utm.my/jurnalteknologi/article/vi...

Abstract

The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized.

Item Type:	Article
Keyword:	Immature embryos , Pigmented upland rice , Shoot regeneration , TDZ pre-treatment , Partial desiccation
Subjects:	S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB113.2-118.46 Seeds. Seed technology
Department:	FACULTY > Faculty of Science and Natural Resources
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	22 Mar 2022 15:06
Last Modified:	22 Mar 2022 15:06
URI:	https://eprints.ums.edu.my/id/eprint/31989

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