In vitro antibacterial activity of marine microalgae extract against vibrio harveyi

Mohd Ridzwan Jusidin and Rafidah Othman and Sitti Raehanah Muhamad Shaleh and Ching Fui Fui @ Faihana Ching Abdullah and Shigeharu Senoo and Siti Nur Hazwani Oslan (2022) In vitro antibacterial activity of marine microalgae extract against vibrio harveyi. Applied Sciences, 12. pp. 1-11. ISSN 2076-3417

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URL: https://www.mdpi.com/2076-3417/12/3/1148/htm

Abstract

Marine microalgae may produce antibacterial substances. At the exponential phase of growth, four species of marine microalgae were examined for their potential to create secondary metabolites that limit the growth of Vibrio harveyi: Nannochloropsis oceanica, Chaetoceros gracilis, Isochrysis sp. and Thalassiosira weissflogii. V. harveyi is a pathogenic bacteria that can cause severe mortality and loss in aquaculture. Disc diffusion assay and co-culture assay were used to determine antibacterial activity. On TSA % NaCl media, the disc impregnated with microalgae and extracted with ethanol, methanol, saline water, and dimethyl sulfoxide (DMSO) was tested against V. harveyi at concentrations of 1.0 × 105 , 106 and 107 CFU mL−1 . The disc diffusion assay revealed that N. oceanica extracted with ethanol had the largest inhibitory zone against V. harveyi. Meanwhile, only N. oceanica, Isochrysis sp. and T. weissflogii reduced the growth of V. harveyi (105 CFU mL−1 ) in the co-culture assay (p < 0.05). The current findings reveal that the hydrophilic chemicals in microalgae extract have antibiotic activity against the highly virulent V. harveyi, which causes vibriosis, a serious disease in farmed fish and aquaculture cultivation around the world.

Item Type:	Article
Keyword:	Microalgae , Antibacterial , Vibrio harveyi , Nannochloropsis oceanica , Chaetoceros gracilis , Isochrysis sp.
Subjects:	Q Science > QK Botany > QK1-989 Botany
Department:	INSTITUTE > Borneo Marine Research Institute
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	25 Apr 2022 11:32
Last Modified:	25 Apr 2022 11:32
URI:	https://eprints.ums.edu.my/id/eprint/32446

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