Cellulase enzyme production from filamentous fungi trichoderma reesei and aspergillus awamori in submerged fermentation with rice straw

Laila Naher and Siti Noor Fatin and Md Abdul Halim Sheikh and Lateef Adebola Azeez and Md. Shafiquzzaman Siddiquee and Norhafizah Md Zain and Sarker Mohammad Rezaul Karim (2021) Cellulase enzyme production from filamentous fungi trichoderma reesei and aspergillus awamori in submerged fermentation with rice straw. Journal of Fungi, 7 (868). pp. 1-11. ISSN 2309-608X

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URL: https://www.mdpi.com/2309-608X/7/10/868/htm

Abstract

Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with Trichoderma reesei, and the weed leaf colonies matched with Aspergillus awamori. These species were coded as T. reesei UMK04 and A. awamori UMK02, respectively. This is the first report of A. awamori UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that T. reesei produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with A. awamori (15.19 U/mg of dry weight), and the concentration was significantly different (p < 0.05). Our results imply that T. reesei can be utilized for cellulase production using rice straw.

Item Type:	Article
Keyword:	Cellulase enzyme , Aspergillus awamori , Trichoderma reesei , Rice straw , Fermentation
Subjects:	Q Science > QK Botany > QK1-989 Botany > QK504-(638) Cryptogams
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	12 Jul 2022 21:24
Last Modified:	12 Jul 2022 21:24
URI:	https://eprints.ums.edu.my/id/eprint/33153

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