An improved protocol for high quantity and quality of genomic DNA isolation from human peripheral blood

Chong, Eric Tzyy Jiann and Goh, Lucky Poh Wah and Png, Keh Kheng and Lee, Ping Chin (2021) An improved protocol for high quantity and quality of genomic DNA isolation from human peripheral blood. Current Applied Science and Technology, 21. pp. 445-455. ISSN 2586-9396

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Abstract

DNA isolation is the most essential step in molecular studies. The quantity and quality of the isolated DNA may subsequently influence the reliability and reproducibility of experimental data especially those involving downstream analysis such as polymerase chain reaction (PCR). In this study, we report an improved protocol for isolating high quantity and quality of genomic DNA from human peripheral blood that is as competitive to commercial kits. The concentration of the genomic DNA isolated using the improved protocol was >100 ng/µl and the A260/A280 absorbance ratio was ranged within 1.604-1.861. When the DNA integrity was measured using Fragment AnalyzerTM, the isolated genomic DNA was highly intact with a genomic quality number of ≥7.0. The isolated genomic DNA was adequate for further molecular analyses including standard PCR and real-time PCR. More importantly, the improved protocol is able to isolate the genomic DNA of Plasmodium parasites that infected human red blood cells, thus enabling them to be correctly identified up to the species level using multiplex PCR.

Item Type:	Article
Keyword:	DNA isolation , Human blood , Polymerase chain reaction , Plasmodium parasites
Subjects:	Q Science > QH Natural history > QH301-705.5 Biology (General) R Medicine > RB Pathology > RB1-214 Pathology
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SAFRUDIN BIN DARUN -
Date Deposited:	16 Jul 2022 17:59
Last Modified:	16 Jul 2022 17:59
URI:	https://eprints.ums.edu.my/id/eprint/33242

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