The Effect of Maturity and Extraction Solvents on Bioactive Compounds and Antioxidant Activity of Mulberry (Morus alba) Fruits and Leaves

Centhyea Chen and Ruzaidi Azli Mohd Mokhtar and Muhamad Shirwan Abdullah Sani and Nor Qhairul Izzreen Mohd Noor (2022) The Effect of Maturity and Extraction Solvents on Bioactive Compounds and Antioxidant Activity of Mulberry (Morus alba) Fruits and Leaves. Molecules, 27 (2406). pp. 1-20. ISSN 1420-3049

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URL: https://www.mdpi.com/1420-3049/27/8/2406

Abstract

Cultivation location, maturity levels, and extraction solvents could affect the bioactive compounds and biological activities of mulberry (Morus alba Linnaeus). The lack of study on Malaysia grown mulberry causes its underutilization. This study investigated the bioactive compound content and the antioxidant activity of Sabah-grown mulberry at two different maturity stages (fruits: red mature and black fully ripe; leaves: young and mature) extracted using 70% (v/v) methanol, 60% (v/v) ethanol, and 65% (v/v) acetone. Analyses showed that mulberry fruits demonstrated maturity dependent increment (except UHPLC-DAD quantification), while the leaves revealed maturity dependent reduction. Principal component analysis (PCA) displayed 65% (v/v) acetone black fully ripe fruits as the best phenolics and antioxidant sources. However, the 60% (v/v) ethanol black fully ripe fruits contained 20.08–68.43% higher total anthocyanins. Meanwhile, the 65% (v/v) acetone and 70% (v/v) methanol red mature fruits were higher in chlorogenic acid (27.53–47.12%) and rutin (31.42–35.92%) than other fruit extracts, respectively. For leaves, 65% (v/v) acetone young leaves were the best phenolics and antioxidant sources. However, the 60% (v/v) ethanol young leaves possessed greater chlorogenic acid (19.56–74.11%) than other leaf extracts. Overall, Malaysia-grown mulberry is rich in phenolics and antioxidants, suggesting its potential application in food and pharmaceutical products.

Item Type:	Article
Keyword:	Bioactive compounds . Antioxidant activity , UHPLC-DAD , Chlorogenic acid , Rutin , Morus alba Linnaeus , Mulberry fruits , Mulberry leaves , Principal component analysis , PCA
Subjects:	Q Science > QK Botany > QK1-989 Botany Q Science > QK Botany > QK1-989 Botany > QK640-(707) Plant anatomy
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	03 Aug 2022 08:15
Last Modified:	03 Aug 2022 08:15
URI:	https://eprints.ums.edu.my/id/eprint/33651

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