Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves

Muhammad Afzal and Riffat Iqbal and Zahed Mahmood and Basit Zeshan and Javed Iqbal Wattoo (2019) Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves. Pakistan Journal of Agricultural Sciences, 56. pp. 709-713. ISSN 0552-9034 (P-ISSN) , 2076-0906 (E-ISSN)

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Abstract

Psidium guajava leaves are rich source of nutrients, antioxidants, phytoconstituents and biological active compounds. The study was designed to elucidate secondary metabolites like alkaloids, saponins, flavonoids, tannins and glycosides in extracts of guava leaves through Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) by qualitative as well as quantitative procedures. These metabolites were further tested for their antimicrobial potential against two-gram positive (Bacillus subtilis and Staphylococcus aureus) and two-gram negative (Escherichia coli and Pasteurella multocida) bacteria and three pathogenic fungal strains (Asprrgillus niger, Fusarium solani and Aspergillus flavus). GC-MS analysis revealed the presence of major constituents like Ca- Caryophyllene (22.70%), α cubebene (11.2%) and alpha Humulene (5.91%). The ethyl-acetate, methanol, n-hexane and chloroform extracts were tested for antibacterial and antifungal activities against above mentioned microbes. Among all the tested solvent extracts, Chloroform and ethyl acetate extracts of P. guajava demonstrated more sensitivity towards the growth of B. subtilis and P. multocida with MIC of 230±3.02, 316. ±6.2 and 237±5.09 and 288±1.55 μg/ml, respectively. Methanolic extracts showed higher MIC against S. aureus (233±5.51 μg/ml) and E. coli (192±2.05 μg/ml), respectively. The findings of this current study would provide the way to use guava as a potential therapeutic agent to combat antimicrobial and antifungal resistance.

Item Type:	Article
Keyword:	Psidium guava , Secondary metabolites , Phyto-medicines , Antibacterial activities , Antifungal resistance
Subjects:	Q Science > QK Botany > QK1-989 Botany S Agriculture > SB Plant culture > SB1-1110 Plant culture
Department:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	15 Nov 2022 09:34
Last Modified:	15 Nov 2022 09:34
URI:	https://eprints.ums.edu.my/id/eprint/34842

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