Introgression of blast resistance genes into the elite rice variety MR263 through marker-assisted backcrossing

Muhammad M Hasan and Mohd Y Rafii and Mohd Razi Ismail and Maziah Mahmood and Md. Amirul Alam and Harun Abdul Rahim and Mohammad A Malek and Mohammad Abdul Latif (2015) Introgression of blast resistance genes into the elite rice variety MR263 through marker-assisted backcrossing. Journal of the Science of Food and Agriculture, 96. pp. 1297-1305. ISSN 0022-5142

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Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/js...

Abstract

BACKGROUND Blast caused by the fungus Magnaporthe oryzae is a significant disease threat to rice across the world and is especially prevalent in Malaysia. An elite, early-maturing, high-yielding Malaysian rice variety, MR263, is susceptible to blast and was used as the recurrent parent in this study. To improve MR263 disease resistance, the Pongsu Seribu 1 rice variety was used as donor of the blast resistance Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 quantitative trait locus (QTL). The objective was to introgress these blast resistance genes into the background of MR263 using marker-assisted backcrossing with both foreground and background selection. RESULTS Improved MR263-BR-3-2, MR263-BR-4-3, MR263-BR-13-1 and MR263-BR-26-4 lines carrying the Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 QTL were developed using the simple sequence repeat (SSR) markers RM5961 and RM263 (linked to the blast resistance genes and QTL) for foreground selection and a collection of 65 polymorphic SSR markers for background selection in backcrossed and selfed generations. A background analysis revealed that the highest rate of recurrent parent genome recovery was 96.1% in MR263-BR-4-3 and 94.3% in MR263-BR-3-2. CONCLUSION The addition of blast resistance genes can be used to improve several Malaysian rice varieties to combat this major disease.

Item Type:	Article
Uncontrolled Keywords:	Blast resistance , Pi gene , Molecular markers , Rice
Subjects:	Q Science > QK Botany > QK1-989 Botany S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General)
Divisions:	FACULTY > Faculty of Sustainable Agriculture
Depositing User:	SITI AZIZAH BINTI IDRIS -
Date Deposited:	22 Nov 2022 10:59
Last Modified:	22 Nov 2022 10:59
URI:	https://eprints.ums.edu.my/id/eprint/34899

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