Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection

Azali Azlan and Muhammad Amir Yunus and Mardani Abdul Halim and Ghows Azzam (2022) Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection. Biology, 11. pp. 1-12. ISSN 2079-7737

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URL: https://www.mdpi.com/2079-7737/11/10/1536

Abstract

The Asian tiger mosquito, Ae. albopictus, is a highly invasive species that transmits several arboviruses including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV). Although several studies have identified microRNAs (miRNAs) in Ae. albopictus, it is crucial to extend and improve current annotations with both the newly improved genome assembly and the increased number of small RNA-sequencing data. We combined our high-depth sequence data and 26 public datasets to re-annotate Ae. albopictus miRNAs and found a total of 72 novel mature miRNAs. We discovered that the expression of novel miRNAs was lower than known miRNAs. Furthermore, compared to known miRNAs, novel miRNAs are prone to expression in a stage-specific manner. Upon DENV infection, a total of 44 novel miRNAs were differentially expressed, and target prediction analysis revealed that miRNA-target genes were involved in lipid metabolism and protein processing in endoplasmic reticulum. Taken together, the miRNA annotation profile provided here is the most comprehensive to date. We believed that this would facilitate future research in understanding virus–host interactions, particularly in the role of miRNAs.

Item Type:	Article
Keyword:	Aedes albopictus, miRNA, Small RNA-sequencing
Subjects:	R Medicine > RC Internal medicine > RC31-1245 Internal medicine > RC109-216 Infectious and parasitic diseases
Department:	INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User:	DG MASNIAH AHMAD -
Date Deposited:	17 Aug 2023 12:18
Last Modified:	17 Aug 2023 12:18
URI:	https://eprints.ums.edu.my/id/eprint/36545

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