May Honey Ohn (2019) A comparative study on the effectiveness of adjunct online learning tool between conventional and gamified e-learning in enhancing electrocardiogram competency among medical students. Doctoral thesis, Universiti Malaysia Sabah.
![[img]](https://eprints.ums.edu.my/style/images/fileicons/text.png) |
Text
24 PAGES.pdf Download (1MB) |
|
Text
FULLTEXT.pdf Restricted to Registered users only Download (26MB) |
Abstract
Homeotic genes are known to play important roles in the development of fruits and flowers. Most of these genes belong to a large family of regulatory genes that have a characteristic DNA binding domain known as the MADS-box. As a pioneer effort in the study of homeotic genes from banana, a full length MADS-box cDNA from an inflorescence of Musa acuminata var. Berangan designated as MADSJ has been successfully isolated and characterized. The approach of this research was by isolating Poly A+ mRNA from the inflorescence of Pisang Berangan using oligo-dT magnetic beads. First strand cDNA was synthesized using a dT18 anchored primer directly onto those beads. A degenerate primer designed by aligning the sequences of the 180bp MADS domain from known MADS-box genes was used as the forward primer to perform a 3' RACE. This generated a complete coding sequence. To obtain the 5' untranslated region on the N terminal side of the MADS-box domain a 5' RACE was carried out. NCBI BLAST analyses were done on these sequences to confirm authenticity to known MADS-box genes. The PHYLIP package was used to further analyze these sequences. The putative 244 amino acid sequence deduced from this research supporting the MADS-box region and the K domain of the corresponding gene suggests that it is a member of the Type II family. MADSJ belongs to the AGL clade and is a member of the SEPALLA TA subfamily of MADS-box transcription factors.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Keyword: | Electrocardiogram, ECG, Online learning tool |
| Subjects: | R Medicine > R Medicine (General) > R5-920 Medicine (General) > R735-854 Medical education. Medical schools. Research |
| Department: | FACULTY > Faculty of Medicine and Health Sciences |
| Depositing User: | DG MASNIAH AHMAD - |
| Date Deposited: | 08 Feb 2024 15:48 |
| Last Modified: | 08 Feb 2024 15:48 |
| URI: | https://eprints.ums.edu.my/id/eprint/38153 |
Actions (login required)
 |
View Item |