Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids

Zainul Amiruddin Zakaria and Adibah Sahmat and Azfar Hizami Azm and Amal Syahirah Nur Zainol and Maizatul Hasyima Omar and Tavamani Balan and Arifah Abdul Kadir and Syahriel Abdullah and Roro Azizah and Lilis Sulistyorini (2023) Liver protective effect of chloroform extract of Bauhinia purpurea leaves is attributed partly to its antioxidant action and the presence of flavonoids. Pharmaceutical Biology, 61 (1). pp. 1-10. ISSN 13880209

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Context: Bauhinia purpurea L. (Fabaceae) is used in the Ayurvedic system to treat various oxidativerelated ailments (e.g., wounds, ulcers etc.). Therefore, it is believed that the plant also has the potential to alleviate oxidative-related liver damage. Objective: This study elucidates the hepatoprotective activity of chloroform extract of B. purpurea leaves (CEBP) in paracetamol (PCM)-induced liver injury (PILI) rats. Materials and methods: Male Sprague-Dawley rats (n ¼ 6) were pre-treated once daily (p.o.) with CEBP (50–500 mg/kg) for seven consecutive days before being administered (p.o.) a hepatotoxic agent, 3 g/kg PCM. Liver enzyme levels were determined from the collected blood, while the collected liver was used to determine the activity of endogenous antioxidant enzymes and for histopathological examination. CEBP was also subjected to radical scavenging assays and phytochemical analysis. Results: CEBP significantly (p < 0.05) reversed the toxic effect of PCM by increasing the serum levels of AST and ALT, and the activity of endogenous catalase (CAT) and superoxide dismutase (SOD) while reducing the liver weight/body weight (LW/BW) ratio. Other than low TPC value and radical scavenging activity, CEBP had a high antioxidant capacity when evaluated using the oxygen radical absorbance capacity (ORAC) assay. UHPLC-ESI-MS analysis of CEBP showed the presence of flavonoids. Discussion and conclusions: CEBP exerts its hepatoprotective activity through a non-free radical scavenging pathway that involves activation of the endogenous enzymatic antioxidant defense system. Further study is needed to identify the responsible bioactive compounds before the plant can be developed as a future alternative hepatoprotective medicament for clinical use.

Item Type: Article
Keyword: Liver injury; paracetamol intoxication; hepatoprotection; non-free radical scavenging; endogenous antioxidant enzymes system
Subjects: G Geography. Anthropology. Recreation > GT Manners and customs > GT1-7070 Manners and customs (General) > GT2400-3390.5 Customs relative to private life Including children, marriage, eating and drinking, funeral customs, etc.
R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA1190-1270 Toxicology. Poisons
Department: FACULTY > Faculty of Medicine and Health Sciences
Date Deposited: 15 Feb 2024 10:37
Last Modified: 15 Feb 2024 10:37

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