Morphological and genetic discordance in two species of Bornean Leptobrachium (Amphibia, Anura, Megophryidae)

Amir Hamidy and Matsui, Masafumi and Shimada, Tomohiko and Nishikawa, Kanto and Yambun, Paul and Ahmad Sudin and Mirza Dikari Kusrini and Hellen Kurniati (2011) Morphological and genetic discordance in two species of Bornean Leptobrachium (Amphibia, Anura, Megophryidae). Molecular Phylogenetics and Evolution, 61 (3). pp. 904-913. ISSN 1055-7903


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Recent phylogenetic studies of Southeast Asian megophryid Leptobrachium, while clarifying (1) distinct specific status of three Philippine populations and (2) high genetic diversities within Bornean Leptobrachium montanum, posed two questions, (1) relationships and divergence histories of two Philippine species and Bornean Leptobrachium gunungense, and (2) possible discordance between phylogenetically and morphologically defined lineages. In order to solve these questions, and especially reviewing current taxonomy of Bornean species, we estimated the phylogenetic relationships of endemic Bornean species together with their putative relatives from Philippines and Sumatra, using 2451bp sequences of the 12S rRNA, tRNA val, and 16S rRNA of mitochondrial DNA genes. With respect to Leptobrachium hasseltii and Leptobrachium chapaense, lineages from Borneo, Philippines, and Sumatra formed a monophyletic group with Leptobrachium lumadorum from Mindanao as the basal clade, while two other Philippine species from Palawan and Mindoro formed a clade and nested in Bornean lineages. Sister species relationship of the two Philippine species and L. gunungense is not supported, rejecting the hypothesis of Philippine origin of L. gunungense. Phylogeny does not conform to morphological variation, and the topotypic L. montanum and one lineage of Leptobrachium abbotti are genetically very close despite their clear difference in ventral color pattern. Furthermore, each of these species forms a paraphyletic group and contains several lineages, each of which is a candidate of good species. These results clearly indicate that current taxonomy of Bornean species based on several morphological characteristics requires complete revision. Detailed studies on adult and larval morphology, as well as acoustic characteristics, are necessary to evaluate the taxonomic status of all lineages recovered.

Item Type: Article
Keyword: Borneo, Cryptic species, Leptobrachium, MtDNA, Philippines, Sumatra, Mitochondrial DNA, RNA 16S, Transfer RNA, Animal, Article, Base pairing, Borneo, DNA sequence, Frogs and toads, Genetic variability, Genetics, Geography, Histology, mitochondrial gene, Phylogeny, Sequence alignment, Species difference, Statistical model, Anura, Base Pairing, Borneo, DNA, Mitochondrial, Genes, Mitochondrial, Genetic Variation, Geography, Likelihood Functions, Phylogeny, RNA, Ribosomal, 16S, RNA, Transfer, Sequence Alignment, Sequence Analysis, DNA, Species Specificity, Amphibia, Anura, Leptobrachium, Leptobrachium chapaense, Leptobrachium hasseltii, Leptobrachium montanum, Megophryidae
Subjects: Q Science > QL Zoology > QL1-991 Zoology > QL605-739.8 Chordates. Vertebrates > QL640-669.3 Reptiles and amphibians
Q Science > QL Zoology > QL1-991 Zoology > QL750-795 Animal behavior
Department: INSTITUTE > Institute for Tropical Biology and Conservation
Depositing User: ADMIN ADMIN
Date Deposited: 07 Aug 2012 13:19
Last Modified: 30 Jul 2021 16:34

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