The effect of thidiazuron on the proliferation of Phaiaenopsis gigantea protocorm

Lilly Malar Govindasamy (2007) The effect of thidiazuron on the proliferation of Phaiaenopsis gigantea protocorm. Universiti Malaysia Sabah. (Unpublished)


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This study was conducted to determine the effects of coconut water (CW) and Thidiazuron (TDZ) on proliferation of Phaiaenopsis gigantea protocorms. The protocorms used for this study were obtained from previously initiated seed germination. Protocorms was segmented vertically and cultured on NDM basal medium containing different concentrations of TDZ (0, 0.1, 0.2, 0.3 and 1.0mg/L) and coconut water (0% and 15% v/v). The One-way ANOVA showed a significant difference among all the treatments media studied on day 120. Protocorm segments exhibited the highest percentage of proliferation on a medium containing 15% (v/v) CW and 0.1mg/L TDZ (68.00 ± 16.43) with an average range of 3-30 new protocorms formed per protocorm segment. Protocorm cultured on a medium containing 15% (v/v) CW and 1.0mg/L TDZ produced the highest number of new protocorms in the range of 0-40 per segment with an average percentage of proliferation 20.00 ± 21.21. Among the concentrations, the best protocorm proliferation was obtained in the combination of 15% (v/v) CW and low concentration (0.lmg/L) of hormone TDZ.

Item Type: Academic Exercise
Keyword: Thidiazuron, Proliferation, Phaiaenopsis gigantea protocorm, Coconut water, Thidiazuron
Subjects: Q Science > QK Botany > QK1-989 Botany > QK474.8-495 Spermatophyta. Phanerogams > QK495 Angiosperms
Department: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 31 Jul 2012 08:04
Last Modified: 23 Oct 2017 14:40

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