Mohammad Amzad Hossain and Muhammad Dawood Shah and Charles Gnanaraj and Muhammad Zafar Iqbal (2011) In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pacific Journal of Tropical Medicine, 4 (9). pp. 717-721. ISSN 1995-7645
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Abstract
Objective: To detect the in vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Methods: The dry powder leaves of Tetrastigma were extracted with different organic solvent such as hexane, ethyl acetate, chloroform, butanol and aqueous methanol. The total phenolic and total flavonoids contents of the essential oil and various organic extracts such as hexane, ethyl acetate, chloroform, butanol and aqueous ethanol were determined by Folin - Ciocalteu method and the assayed antioxidant activity was determined in vitro models such as antioxidant capacity by radical scavenging activity using α, α-diphenyl- β-picrylhydrazyl (DPPH) method. Results: The total phenolic contents of the essential oil and different extracts as gallic acid equivalents were found to be highest in methanol extract (386.22 mg/g) followed by ethyl acetate (190.89 mg/g), chloroform (175.89 mg/g), hexane (173.44 mg/g), and butanol extract (131.72 mg/g) and the phenolic contents not detected in essential oil. The antioxidant capacity of the essential oil and different extracts as ascorbic acid standard was in the order of methanol extract > ethyl acetate extract >chloroform> butanol > hexane extract also the antioxidant activity was not detected in essential oil. Conclusions: The findings show that the extent of antioxidant activity of the essential oil and all extracts are in accordance with the amount of phenolics present in that extract. Leaves of Tetrastigma being rich in phenolics may provide a good source of antioxidant.
| Item Type: | Article |
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| Keyword: | Antioxidant activity, Essential oil, Extracts, Flavonoids, Tetrastigma, Total phenolics, 1,1 diphenyl 2 Picrylhydrazyl, Acetic acid ethyl ester, Antioxidant, Ascorbic acid, Butanol, Chloroform, Essential oil, Flavonoid, Gallic acid, Hexane, Methanol, Organic solvent, Phenol derivative, Plant extract, Scavenger, Tetrastigma essential oil, Tetrastigma extract, Unclassified drug, Antioxidant activity, Drug determination, Drug isolation, Drug screening, Dry powder, In vitro study, Malaysia, Medicinal plant, Nonhuman, Plant leaf, Priority journal, Tetrastigma, Antioxidants, Flavonoids, Malaysia, Oils, Volatile, Phenols, Plant Extracts, Plant Leaves, Vitaceae |
| Subjects: | T Technology > TP Chemical technology > TP1-1185 Chemical technology > TP248.13-248.65 Biotechnology S Agriculture > SB Plant culture |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 01 Oct 2012 17:52 |
| Last Modified: | 20 Oct 2017 12:51 |
| URI: | https://eprints.ums.edu.my/id/eprint/4988 |
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