Noor Hydayaty Md. Yusuf and Vijay Kumar Subbiah (2011) Identification and characterization of differentially expressed microRNAs during fruit ripening in pineapple (Ananas comosus var. comosus). Acta Horticulturae, 902. pp. 123-132. ISSN 0567-7572
Full text not available from this repository.Abstract
MicroRNAs (miRNAs) are a class of small, non-coding, endogenous RNAs that negatively regulates gene expression by cleaving or repressing the translational process of protein-coding mRNA transcripts. To determine if pineapple fruit development and ripening was mediated by miRNA, miRNA were detected and quantified at pre- and post-ripening stages using RT-qPCR. The expression profiles of 13 miRNAs namely miR156, miR157, miR159, miR162, miR164, miR165, miR167, miR168, miR169, miR170, miR171, miR172, and miR390 were detected in pineapple. Among these 13 miRNAs, only the genes (miR168, miR390, and miR164) displayed clear differential expression during pre-ripening and post-ripening stages of the fruit. During the post-ripening stage, miR168 and miR390 were up-regulated 1.79- and 2.64-fold, respectively, while expression of miR164 dropped to 0.07-fold compared to the pre-ripening stage. The discovery of miRNAs from this study together with its stage-regulated expression profiles are excellent candidates for further investigation into pineapple crop improvement through functional genomes.
| Item Type: | Article |
|---|---|
| Keyword: | Fruit ripening, MicroRNA, Non-climacteric fruit, Pineapple, RT-qPCR |
| Subjects: | S Agriculture > SB Plant culture > SB1-1110 Plant culture > SB354-402 Fruit and fruit culture |
| Department: | INSTITUTE > Biotechnology Research Institute (BRI) |
| Depositing User: | ADMIN ADMIN |
| Date Deposited: | 05 Oct 2012 14:46 |
| Last Modified: | 08 Sep 2014 11:56 |
| URI: | https://eprints.ums.edu.my/id/eprint/5025 |
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