Characterization of Malaysian Trichoderma isolates using the Random Amplified Microsatellites (RAMS). Molecular Biology

Shafiquzzaman Siddiquee and Soon, Guan Tan and Umi Kalsom Yusuf and Nur Hasan Nudin Fatihah and Md. Mainul Hasan (2012) Characterization of Malaysian Trichoderma isolates using the Random Amplified Microsatellites (RAMS). Molecular Biology. Molecular Biology Reports, 29 (1). pp. 715-722. ISSN 0301-4851


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Trichoderma species are commercially appliedas biocontrol agents against numerous plant pathogenicfungi due to their production of antifungal metabolites,competition for nutrients and space, and mycoparasitism.However, currently the identi�cation of Trichoderma spe-cies from throughout the world based on micro-morpho-logical descriptions is tedious and prone to error. Thecorrect identi�cation of Trichoderma species is importantas several traits are species-speci�c. The Random Ampli-�ed Microsatellites (RAMS) analysis done using �veprimers in this study showed different degrees of the genetic similarity among 42 isolates of this genus. Thegenetic similarity values were found to be in the range of 12.50–85.11% based on a total of 76 bands scored in the Trichoderma isolates. Of these 76 bands, 96.05% werepolymorphic, 3.95% were monomorphic and 16% wereexclusive bands. Two bands (250 bp and 200 bp) producedby primer LR-5 and one band (250 bp) by primer P1Awere present in all the Trichoderma isolates collected fromhealthy and infected oil palm plantation soils. Clusteranalysis based on UPGMA of the RAMS marker datashowed that T. harzianum, T. virens and T. longibrachia-tum isolates were grouped into different clades and lin-eages. In this study we found that although T. aureoviride isolates were morphologically different when compared to T. harzianum isolates, the UPGMA cluster analysis showedthat the majority isolates of T. aureoviride (seven fromnine) were closely related to the isolates of T. harzianum.

Item Type: Article
Keyword: Trichoderma, Random ampli�edmicrosatellites (RAMS), Cluster analysis
Subjects: Q Science > QH Natural history > QH301-705.5 Biology (General)
Department: INSTITUTE > Biotechnology Research Institute (BRI)
Depositing User: ADMIN ADMIN
Date Deposited: 08 Jan 2013 08:32
Last Modified: 11 Oct 2017 09:41

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